Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system

• Jing Hu,
• Chris Weise,
• Christoph Böttcher,
• Hua Fan and
• Jian Yin

Beilstein J. Org. Chem. 2017, 13, 874–882, doi:10.3762/bjoc.13.88

• Avenue1800, Wuxi 214122, China 10.3762/bjoc.13.88 Abstract The γ-aminobutyric acid (GABA) transporter 1 (GAT1) belongs to a family of Na+ and Cl−-coupled transport proteins and possesses 12 putative transmembrane domains. To perform structural analyses of the GAT1 protein, the GAT1/green fluorescent protein

• isolated in its monomeric form. Keywords: baculovirus expression system; chromatography; γ-aminobutyric acid (GABA); GABA transporter 1 (GAT1); Introduction γ-Aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the central nervous system (CNS) of vertebrate species. GABAergic